| Online ISSN: 1096-9861 | Print ISSN: 0021-9967 |
The Journal of Comparative Neurology
Volume 505 Issue 2 • Pages 190–208
DOI: 10.1002/cne.21473
Published online: 17 Sep 2007
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The Journal of Comparative Neurology Volume 505 Issue 2 • Pages 190–208 DOI: 10.1002/cne.21473
Published online: 17 Sep 2007
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| Copyright © 2007 Wiley-Liss, Inc., A Wiley Company |
| File | Format | Size | Description |
|---|---|---|---|
| Supporting information | QuickTime video file | 8 Mb | Movie 1 Dcx-eGFP arrays in the SVZ imaged for 8:53. Notice the robust migration in the longitudinal arrays and that the arrays do not change shape over several hours. Corresponds to Fig. 4A-I. |
| Supporting information | QuickTime video file | 2 Mb | Movie 2 Dcx-eGFP arrays in the SVZ. Notice that cells do not extend processes away from the longitudinal array of cells. The bright cluster of cells at the lower right is an array cut in cross section. Dorsally, several cells at the edge of the arrays appear to be stationary. |
| Supporting information | QuickTime video file | 3 Mb | Movie 3 Dcx-eGFP migration in the RMS as it enters the OB. Robust rostrocaudal migration is observed in multiple arrays of cells. In addition, many stationary Dcx-eGFP cells are seen, primarily at the edge of the RMS. Corresponds to Fig. 4J-M. |
| Supporting information | QuickTime video file | 3 Mb | Movie 4 Gad-eGFP cells in the SVZ. The majority of migration in this movie is rostrocaudal and directly beneath the corpus callosum. This slice included a portion of the striatal SVZ; cells in this region migrate dorsoventrally and in a variety of directions. |
| Supporting information | QuickTime video file | 4 Mb | Movie 5 Gad-eGFP cells in the SVZ. Note that striatal Gad-eGFP cells retain fluorescence throughout the imaging. Corresponds to Fig. 7A-H. |
| Supporting information | QuickTime video file | 952 Kb | Movie 6 Gad-eGFP cells in the SVZ imaged for 2:13. Corresponds to Fig. 5G-K. |
| Supporting information | QuickTime video file | 6 Mb | Movie 7 Nestin-eGFP cells in the SVZ imaged for 7:21. This movie contains cells that are motile in an exploratory fashion; they move stochastically, do not exhibit long net movements, and have highly labile processes. Corresponds to Fig. 8A-J and the second set of traces shown in Mov. 11. Movie 7 Nestin-eGFP cells in the SVZ imaged for 7:21. This movie contains cells that are motile in an exploratory fashion; they move stochastically, do not exhibit long net movements, and have highly labile processes. Corresponds to Fig. 8A-J and the second set of traces shown in Mov. 11. |
| Supporting information | QuickTime video file | 2 Mb | Movie 8 Nestin-eGFP cells in the RMS imaged for 2:09. Cells moved faithfully within the RMS. Note the cell in the upper left part of the frame that migrates ventrally out of the RMS only to rapidly change directions and rejoin the stream. |
| Supporting information | QuickTime video file | 3 Mb | Movie 9 Nestin-eGFP cells in the RMS imaged for 2:36. Note the bright cell at the top left that has migratory morphology (long leading process) but that remains stationary (Fig. 6B). Also note the significant number of cells moving caudally. |
| Supporting information | QuickTime video file | 4 Mb | Movie 10 Nestin-eGFP cells in the RMS imaged for 3:30. Corresponds to Fig. 9. |
| Supporting information | QuickTime video file | 12 Mb | Movie 11 4D plots of cell movement. The green dots indicate the original position and the red dots the final position of cells. a. GAD-eGFP; corresponds to Fig. 7. The majority of cells moved long distances rostrally. Cell #7 migrated caudally and cell #6 was exploratory. The traces are followed by a vectorial analysis wherein start positions are normalized, distance and direction mapped, and averaged. Note that the vector average (dark blue line) points to the olfactory bulb. b. Nestin-eGFP; corresponds to Mov. 7 and Fig. 8A-J, with 6 additional cells. Multiple patterns of migration occur in this movie. Viewing patterns of migration from multiple angles shows that small subregions of the SVZ contain a variety of migratory patterns. Please focus on the ∼1.3x106 μm3 volume containing the orange, purple, and light blue pathways and note that cells #4 and 6 migrate countercurrent to each other, whereas the pattern of movement of cell #1 is completely different. A few cells ex. #7 (light pink trace) moved primarily in the Z-dimension causing apparent movement in Quicktime movies to appear artificially minimal. The mediolateral movement is only evident when traces are viewed from the side. (Cell "#12" is a retrace of cell #2 which confirms the accuracy of our methodology.) Cell #9 is a good example of a cell that migrates caudally and then turns back to migrate rostrally. |
| Supporting information | TIFF image file | 4 Mb | Suppl. Table 1 List of movies made from Dcx-eGFP, Gad-eGFP, and nestin-eGFP mice. The movies are listed numerically by postnatal age, duration of imaging (hrs:mins), and area imaged. Schematics show the locations of imaged areas for each group. |
| Supporting information | TIFF image file | 8 Mb | Suppl. Fig. 1 First frames of Dcx-eGFP and Gad-eGFP slices used in study. Locations of imaging is shown in schematics for each slice. Slice numbers included in schematics. Movies from all slices available upon request. |
| Supporting information | TIFF image file | 6 Mb | Suppl. Fig. 2 First frames of nestin-eGFP slices used in study. Locations of imaging is shown in schematics for each slice. Slice numbers included in schematics. Movies from all slices available upon request. |