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Encyclopedia of Life Sciences

Animal Cell Separation and Fractionation introductory

Standard Article

R Curtis Bird, Auburn University, Auburn, Alabama, USA
Allison E Church Bird, Auburn University, Auburn, Alabama, USA
Patricia DeInnocentes, Auburn University, Auburn, Alabama, USA

DOI: 10.1038/npg.els.0002588
Article Online Posting Date: May 3, 2005

Abstract | Full Text: HTML PDF (110K)

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Abstract

Many cell types containing many different compartments can be fractionated into special cell populations or subcellular compartments. Fractionation strategies must be tailored to provide the highest quality enrichment of the fraction of cells, organelles or complexes sought and after careful assessment of the target and the uses to which the final enriched fraction will be put.

Keywords: cell separation; cell fractionation; cell compartments; organelles; fibroblast depletion